Redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy Conference Paper uri icon


  • 2017 SPIE. Resonance Raman spectroscopy of cytochrome c was used to follow reduction/oxidation (redox) states of isolated mitochondria in response to blue or red laser exposure. Mitochondria were isolated from hTERT-RPE1 cells and were kept in a buffer formulation known to be conducive to electron transport chain (ETC) activity. Using either pyruvate or succinate as substrates for ETC, we found differences in the redox responses of cytochrome c for different exposure laser irradiance and excitation wavelength. We anticipate that the proposed new method will be valuable in the study of metabolic processes in mitochondria in response to low level laser exposure, and thus aid in elucidating the mechanism(s) of photobiomodulation.

name of conference

  • Mechanisms of Photobiomodulation Therapy XIII

published proceedings


author list (cited authors)

  • Denton, M. L., Gonzalez, C. C., Noojin, G. D., & Yakovlev, V. V.

citation count

  • 4

complete list of authors

  • Denton, Michael L||Gonzalez, Cherry C||Noojin, Gary D||Yakovlev, Vladislav V

editor list (cited editors)

  • Hamblin, M. R., Carroll, J. D., & Arany, P.

publication date

  • February 2018