Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Academic Article

abstract

• Intact, viable X and Y chromosome-bearing sperm populations of the rabbit were separated according to DNA content with a flow cytometer/cell sorter. Reanalysis for DNA of an aliquot from each sorted population showed purities of 86% for X-bearing sperm and 81% for Y-bearing sperm populations. Sorted sperm were surgically inseminated into the uterus of rabbits. From does inseminated with sorted X-bearing sperm, 94% of the offspring born were females. From does inseminated with sorted Y-bearing sperm from the same ejaculates, 81% of the offspring were males. The probability of the phenotypic sex ratios differing from 50:50 were p less than 0.0003 for X-sorted sperm and p less than 0.004 for Y-sorted sperm. Thus, the phenotypic sex ratio at birth was accurately predicted from the flow-cytometrically measured proportion of X- and Y-bearing sperm used for insemination.

authors

• Johnson, Larry

published proceedings

• Biol Reprod

altmetric score

• 15

author list (cited authors)

• Johnson, L. A., Flook, J. P., & Hawk, H. W.

citation count

• 283

complete list of authors

• Johnson, LA||Flook, JP||Hawk, HW

publication date

• August 1989

publisher

• Oxford University Press (OUP)  Publisher

published in

• Biology of Reproduction  Journal

keywords

• Animals
• Cell Separation
• DNA
• Female
• Fertilization
• Flow Cytometry
• Genetic Engineering
• Insemination, Artificial
• Male
• Phenotype
• Pregnancy
• Rabbits
• Sex Preselection
• Sex Ratio
• Spermatozoa
• X Chromosome
• Y Chromosome

PubMed Central ID

• 2804212

Digital Object Identifier (DOI)

• 10.1095/biolreprod41.2.199

start page

• 199

end page

• 203

volume

• 41

issue

• 2

URL

• http://dx.doi.org/10.1095/biolreprod41.2.199