Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Academic Article uri icon


  • Intact, viable X and Y chromosome-bearing sperm populations of the rabbit were separated according to DNA content with a flow cytometer/cell sorter. Reanalysis for DNA of an aliquot from each sorted population showed purities of 86% for X-bearing sperm and 81% for Y-bearing sperm populations. Sorted sperm were surgically inseminated into the uterus of rabbits. From does inseminated with sorted X-bearing sperm, 94% of the offspring born were females. From does inseminated with sorted Y-bearing sperm from the same ejaculates, 81% of the offspring were males. The probability of the phenotypic sex ratios differing from 50:50 were p less than 0.0003 for X-sorted sperm and p less than 0.004 for Y-sorted sperm. Thus, the phenotypic sex ratio at birth was accurately predicted from the flow-cytometrically measured proportion of X- and Y-bearing sperm used for insemination.

published proceedings

  • Biol Reprod

altmetric score

  • 15

author list (cited authors)

  • Johnson, L. A., Flook, J. P., & Hawk, H. W.

citation count

  • 283

complete list of authors

  • Johnson, LA||Flook, JP||Hawk, HW

publication date

  • August 1989