Distribution of glutamic oxalacetic transaminase and lactic dehydrogenase activities in boar semen after cold shock and freezing. Academic Article uri icon

abstract

  • The glutamic oxalacetic transaminase (GOT) and lactic dehydrogenase (LDH) activities of spermatozoa and seminal plasma were determined for control (25 C), cold-shocked (0 C), and deep frozen (-196 C) boar semen. Semen from four ejaculates of each of four boars was used. Freezing caused significant (P < 0.01) losses of GOT and LDH activities from the spermatozoa and concomitant significant (P < 0.01) increases in the seminal plasma. The magnitude of enzyme losses from spermatozoa was greater for GOT (68.7 to 86.2%) than for LDH (22.6 to 31.8%). In addition to "leaching" of enzymes from sperm cells into seminal plasma, some inactivation of LDH occurred during freezing. Cold shock caused significant (P < 0.05) losses of GOT and LDH activities from the spermatozoa. Dilution of boar semen with an egg yolk extender slightly reduced the loss of GOT and LDH activities occurring during cold shock. The data indicate that GOT activity may be a more useful parameter than LDH activity for assessing boar sperm cell damage during freezing. 1970.

published proceedings

  • Cryobiology

author list (cited authors)

  • Pursel, V. G., Johnson, L. A., & Gerrits, R. J.

citation count

  • 15

complete list of authors

  • Pursel, VG||Johnson, LA||Gerrits, RJ

publication date

  • September 1970