Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development.

abstract

• Media are available that can deliver modest porcine embryonic development from a single-cell zygote to the blastocyst stage. However, few embryos develop to hatched blastocysts by Day 7 in vitro, indicating deficiencies in media that inhibit early embryonic development. A defined culture medium, Beltsville Embryo Culture Medium (BECM-3), was developed to support porcine zygote development to the blastocyst stage. When fetal bovine serum was added by late Day 5 (late morula/early blastocyst stage), 80% of total embryos cultured from Day 2 developed into hatched blastocysts by Day 8. There was also a significant increase in the mean cell number of blastocysts and hatched blastocysts when culture was performed in BECM-3-based media in the absence of BSA fraction V. These studies provide a chemically defined foundation for elucidating key developmental components of preimplantation pig embryos.

authors

• Johnson, Larry

published proceedings

• Biol Reprod

altmetric score

• 3

author list (cited authors)

• Dobrinsky, J. R., Johnson, L. A., & Rath, D.

citation count

• 119

complete list of authors

• Dobrinsky, JR||Johnson, LA||Rath, D

publication date

• November 1996

publisher

• Oxford University Press (OUP)  Publisher

published in

• Biology of Reproduction  Journal

keywords

• Animals
• Blastocyst
• Cattle
• Culture Media
• Culture Techniques
• Embryonic And Fetal Development
• Fetal Blood
• Morula
• Serum Albumin, Bovine
• Swine
• Zygote

PubMed Central ID

• 8902219

Digital Object Identifier (DOI)

• 10.1095/biolreprod55.5.1069

start page

• 1069

end page

• 1074

volume

• 55

issue

• 5

URL

• http://dx.doi.org/10.1095/biolreprod55.5.1069