An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures. Academic Article uri icon


  • X and Y chromosome paints were developed from sorted yak chromosomes for sexing cattle spermatozoa. Clear hybridization signals were obtained for every spermatozoon using a modified sperm decondensation protocol and fluorescence in situ hybridization (FISH). The procedure was evaluated using the established Beltsville sperm sexing technology, which separates spermatozoa by flow cytometry into X- and Y-bearing fractions. Close agreement was found between the assessment of sperm separation by flow cytometry and by FISH with the X-Y paint set. The FISH method is a simple, reliable and robust procedure for assessing the effectiveness of separation of X and Y spermatozoa.

published proceedings

  • Reproduction

altmetric score

  • 3

author list (cited authors)

  • Rens, W., Yang, F., Welch, G., Revell, S., O'Brien, P. C., Solanky, N., Johnson, L. A., & Ferguson Smith, M. A.

citation count

  • 43

complete list of authors

  • Rens, W||Yang, F||Welch, G||Revell, S||O'Brien, PC||Solanky, N||Johnson, LA||Ferguson Smith, MA

publication date

  • April 2001