An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures. Academic Article

abstract

• X and Y chromosome paints were developed from sorted yak chromosomes for sexing cattle spermatozoa. Clear hybridization signals were obtained for every spermatozoon using a modified sperm decondensation protocol and fluorescence in situ hybridization (FISH). The procedure was evaluated using the established Beltsville sperm sexing technology, which separates spermatozoa by flow cytometry into X- and Y-bearing fractions. Close agreement was found between the assessment of sperm separation by flow cytometry and by FISH with the X-Y paint set. The FISH method is a simple, reliable and robust procedure for assessing the effectiveness of separation of X and Y spermatozoa.

authors

• Johnson, Larry

published proceedings

• Reproduction

altmetric score

• 3

author list (cited authors)

• Rens, W., Yang, F., Welch, G., Revell, S., O'Brien, P. C., Solanky, N., Johnson, L. A., & Ferguson Smith, M. A.

citation count

• 43

complete list of authors

• Rens, W||Yang, F||Welch, G||Revell, S||O'Brien, PC||Solanky, N||Johnson, LA||Ferguson Smith, MA

publication date

• April 2001

publisher

• Bioscientifica  Publisher

published in

• REPRODUCTION  Journal

keywords

• Animals
• Cattle
• Cell Separation
• Cells, Cultured
• Chromosome Painting
• DNA
• Flow Cytometry
• In Situ Hybridization, Fluorescence
• Male
• Sex Determination Analysis
• Spermatozoa
• X Chromosome
• Y Chromosome

PubMed Central ID

• 11277872

Digital Object Identifier (DOI)

• 10.1530/rep.0.1210541

start page

• 541

end page

• 546

volume

• 121

issue

• 4

URL

• http://dx.doi.org/10.1530/rep.0.1210541