Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation. Academic Article

abstract

• The acrosome morphology, as assessed by phase-contrast microscopy, was not altered by fixing boar spermatozoa with 0.1 to 2% glutaraldehyde in comparison to spermatozoa inhibited with NaF. The acrosome morphology of glutaraldehyde-fixed samples remained unchanged for 14 days. Boar semen diluted and stored in Beltsville L1 (BL1), egg-yolk-glucose-bicarbonate (EGB), and Beltsville F5 (BF5) could be fixed with glutaraldehyde for acrosome evaluation if the supernatant solution containing egg yolk was removed immediately after glutaraldehyde addition. Spermatozoa extended in Beltsville F3 (BF3) extender could not be microscopically evaluated after glutaraldehyde fixation because of casein precipitation. Acrosome morphology of glutaraldehyde-fixed boar spermatozoa remained unchanged for at least 19 days holding at room temperature or shipping by parcel post. 1974.

authors

• Johnson, Larry

published proceedings

• Theriogenology

author list (cited authors)

• Pursel, V. G., & Johnson, L. A.

citation count

• 144

complete list of authors

• Pursel, VG||Johnson, LA

publication date

• February 1974

publisher

• Elsevier  Publisher

published in

• THERIOGENOLOGY  Journal

keywords

• Aldehydes
• Animals
• Cell Survival
• Glutaral
• Male
• Microscopy, Phase-Contrast
• Specimen Handling
• Spermatozoa
• Swine

PubMed Central ID

• 4219847

Digital Object Identifier (DOI)

• 10.1016/0093-691x(74)90008-9

start page

• 63

end page

• 68

volume

• 1

issue

• 2

URL

• http://dx.doi.org/10.1016/0093-691x(74)90008-9