Modification of a laser-based flow cytometer for high-resolution DNA analysis of mammalian spermatozoa. Academic Article

abstract

• Modification of a Coulter EPICS V orthogonal laser-based flow cytometer/cell sorter allows resolution of X and Y mammalian sperm populations based on DNA content. The modification consists of beveling the sample injection tube situated in the flow chamber, adding a second fluorescence detector directly forward along the laser beam axis, and routing the collected fluorescence through an optical fiber bundle to one of the existing photomultiplier tubes. The X and Y chromosome-bearing spermatozoa from the bull, boar, and ram can be resolved using this system.

authors

• Johnson, Larry

published proceedings

• Cytometry

altmetric score

• 9

author list (cited authors)

• Johnson, L. A., & Pinkel, D.

citation count

• 116

complete list of authors

• Johnson, LA||Pinkel, D

publication date

• May 1986

publisher

• Wiley  Publisher

published in

• Cytometry  Journal

keywords

• Animals
• Cattle
• Cell Nucleus
• Cell Separation
• DNA
• Evaluation Studies As Topic
• Flow Cytometry
• Lasers
• Male
• Sheep
• Spermatozoa
• Swine

PubMed Central ID

• 3709306

Digital Object Identifier (DOI)

• 10.1002/cyto.990070307

start page

• 268

end page

• 273

volume

• 7

issue

• 3

URL

• http://dx.doi.org/10.1002/cyto.990070307