Inability of Flow Cytometry to Distinguish X from YChromosomeBearing Spermatozoa of Chinook Salmon
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A modified flow cytometer was used to look for differences in DNA content between X- and Y-chro- mosome-bearing spermatozoa of Chinook salmon (On- corhynchus tshawytscha). This system has been used successfully to make such determinations in several mammalian species, but it could neither distinguish between X- and Y-chromosome-bearing spermatozoa in sperm from heterogametic males, nor between total DNA content of sperm from heterogametic and homogametic (hormonally masculinized) males. 1991 Taylor & Francis Group, LLC.