Polymerase Chain Reaction-Based Sex Identification in the Greater Roadrunner Academic Article uri icon


  • We determined a polymerase chain reaction (PCR)based method to identify the sex of greater roadrunners (Geococcyx californianus). We found that previously reported primer combinations used in other bird species did not work reliably in roadrunners. However, a novel primer combination (P2-1237L) allowed for consistent and reliable sex identification of roadrunners. Conducting several PCR replicates per sample helped to identify occasional ?referential amplification of the Z band that could occur in females (the heterogametic sex; i.e., WZ). Identifying the sex of individuals will allow for examination of sex-associated differences in home range size and habitat use, population demographics, and parenting behavior, as well as for a better understanding of the social system of roadrunners. 2010 The Wildlife Society.

published proceedings


author list (cited authors)

  • Santamaria, C. A., Kelley, S., Schulz, G. G., Ransom, D. J., & Hurtado, L. A.

citation count

  • 1

complete list of authors

  • Santamaria, Carlos A||Kelley, Samuel||Schulz, Gerral G||Ransom, Dean Jr||Hurtado, Luis A

publication date

  • August 2010