Diversity of nitrogen isotopes and protein status in caribou: implications for monitoring northern ungulates
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Nutritional condition is an important determinant of productivity and survival in caribou (Rangifer tarandus). We used samples of excreta (n 1,150) to estimate diet composition from microhistology and 2 isotopic proxies of protein status for 2 ecotypes of caribou in 4 herds in late winter (20062008). Isotopes of nitrogen (15N in parts per thousand ‰) from excreta samples (urea, diet, and body N) were used to estimate indexes of protein status: the proportion of urea N derived from body N (p-UN) and the difference between the 15N of the body and urinary urea (δ body-urea). We examined dietary and terrain characteristics, 15N, p-UN, and δbody-urea by ecotype, herd, year, and foraging site. Multiple regression and an information-theoretic approach were used to evaluate correlates of protein status at each foraging site. The dietary and terrain characteristics of foraging sites did not vary by ecotype or herd (P > 0.108); diets were dominated by lichens (68 ± 14.1 SD). The 15N of urea, diet, body N, p-UN, and δbody-urea varied among foraging sites within each herd (P < 0.001). Although highly variable, the 15N of urinary urea was typically low (-4.68‰ ± 2.67‰ SD). Dietary N also had low 15N (-4.18‰ ± 0.92‰ SD), whereas body N was generally heavier in 15N (2.20‰ ± 1.56‰ SD) than urinary urea or the diet. Both measures of protein status were similarly diverse between ecotypes and among herds, which limited their applicability to monitor protein status at the population level. Although we observed limitations to interpreting estimates of p-UN from highly vagile ungulates, the δbody-urea may prove to be a useful index of protein status at smaller spatial and temporal scales. Indeed, a portion of the observed variance (r2 0.26) in δbody-urea at each foraging site was explained by the proportion of shrubs in the winter diet. There remains potential in using 15N in excreta as a noninvasive tool for evaluating protein status in northern ungulates; however, considerable analytical and sampling challenges remain for applying these isotopic approaches at large scales. © 2012 American Society of Mammalogists.
author list (cited authors)
Gustine, D. D., Barboza, P. S., Lawler, J. P., Adams, L. G., Parker, K. L., Arthur, S. M., & Shults, B. S.