Genomic DNA Libraries and Physical Mapping Chapter uri icon

abstract

  • The bacteria-based large-insert genomic DNA cloning system has become the most widely used cloning system for genome physical mapping due to its large cloning capacity, stable maintenance of large-insert genomic DNA in Escherichia coli, and ease of manipulation. The advent of the bacteria-based large-insert cloning system has been attributed to both the development of electroporation transformation technology and the discovery of E. coli host strain DH10B. Qualities of source libraries such as insert size, clone genome coverage, genome representation, and binary vectors have great influence on high-quality genome physical mapping. State-of-the-art techniques for construction of the large-insert genomic DNA libraries in bacteria have been described in detail. The bacteria-based large-insert genomic DNA libraries have widely been applied for physical mapping using a diversity of mapping methods, including fingerprint analysis FISH, optical mapping, iterative hybridization, and other methods. © 2005 WILEY-VCH Verlag GmbH and Co.

author list (cited authors)

  • Ren, C., Xu, Z., Sun, S., Lee, M., Wu, C., Scheuring, C., & Zhang, H.

citation count

  • 20

Book Title

  • The Handbook of Plant Genome Mapping

publication date

  • January 2005