Investigation of seedling-stage salinity tolerance QTLs using backcross lines derived from Oryza sativa L. Pokkali Academic Article uri icon

abstract

  • With 2 figures and 5 tables A population of BC 3 F 4 rice lines derived from a cross of salt-tolerant Pokkali and sensitive IR29 was developed for validation of the Saltol QTL and comparison of this QTL effect with background QTLs. Specific BC 3 F 4 progenies from the advanced backcross population were found to have similar salinity tolerance at the seedling stage even without the Saltol alleles. Therefore, additional QTLs responsible for seedling-stage salinity tolerance were investigated by screening and genotyping with markers at multiple loci throughout the 12 rice chromosomes. Single marker regression (SMR) using Q-Gene detected seven QTLs above the threshold logarithm of the odds of 3.0. Single marker analysis using IciMapping detected the same markers linked to the seven QTLs identified by SMR. Likelihood ratio test-based on stepwise regression (LRT-RSTEP) analysis using IciMapping, more suitable for BC 3 F 4 introgression lines located six QTLs, of which two were common with SMR and included two significant segments within Saltol. The new non-Saltol QTLs represent targets for further study to evaluate their usefulness in breeding for higher levels of seedling-stage salinity tolerance in rice. © 2011 Blackwell Verlag GmbH.

published proceedings

  • Plant Breeding

altmetric score

  • 1.5

author list (cited authors)

  • Alam, R., Rahman, M. S., Seraj, Z. I., Thomson, M. J., Ismail, A. M., Tumimbang‐Raiz, E., & Gregorio, G. B.

citation count

  • 41

complete list of authors

  • Alam, Rebjana||Sazzadur Rahman, M||Seraj, Zeba I||Thomson, Michael J||Ismail, Abdelbagi M||Tumimbang-Raiz, Ellen||Gregorio, Glenn B

publication date

  • August 2011

publisher