Factors affecting ANKOM fiber analysis of forage and browse varying in condensed tannin concentration. Academic Article uri icon


  • BACKGROUND: Browse species containing condensed tannins (CTs) are an important source of nutrition for grazing/browsing livestock and wildlife in many parts of the world, but information on fiber concentration and CT-fiber interactions for these plants is lacking. RESULTS: Ten forage or browse species with a range of CT concentrations were oven dried and freeze dried and then analyzed for ash-corrected neutral detergent fiber (NDFom) and corrected acid detergent fiber (ADFom) using separate samples (ADFSEP) and sequential NDF-ADF analysis (ADFSEQ) with the ANKOM fiber analysis system. The ADFSEP and ADFSEQ residues were then analyzed for nitrogen (N) concentration. Oven drying increased (P < 0.05) fiber concentrations with some species, but not with others. For high-CT forage and browse species, ADFSEP concentrations were greater (P < 0.05) than NDFom values and approximately double the ADFSEQ values. Nitrogen concentration was greater (P < 0.05) in ADFSEP than ADFSEQ residues, likely due to precipitation with CTs. CONCLUSION: Sequential NDF-ADF analysis gave more realistic values and appeared to remove most of the fiber residue contaminants in CT forage samples. Freeze drying samples with sequential NDF-ADF analysis is recommended in the ANKOM fiber analysis system with CT-containing forage and browse species.

published proceedings

  • J Sci Food Agric

author list (cited authors)

  • Terrill, T. H., Wolfe, R. M., & Muir, J. P.

citation count

  • 8

complete list of authors

  • Terrill, Thomas H||Wolfe, Richard M||Muir, James P

publication date

  • December 2010