Sucrose synthase molecular marker associated with sugar content in elite sugarcane progeny Academic Article uri icon

abstract

  • We describe the development and application of an expressed sequence tag (EST)-derived restriction fragment length polymorphism (RFLP) marker for sugarcane elite genotypes which can be used for quantitative trait loci (QTL) tagging for sugar content. EST-derived RFLP markers for proteins involved in sucrose metabolism have been used in Southern analysis for mapping and gene tagging in elite sugarcane clones. A single dose marker, obtained from a sucrose synthase EST associated with sugar content at the α = 0.01 probability level, is presented for sugarcane breeding. Utilization of EST homologues to known genes for generation of molecular markers accelerated the identification of a QTL controlling an important trait-sugar content. Sugarcane bacterial artificial chromosome (BAC) clones hybridizing to the sucrose synthase EST were identified. Copyright by the Brazilian Society of Genetics.

author list (cited authors)

  • da Silva, J. A., & Bressiani, J. A.

citation count

  • 36

publication date

  • January 2005

keywords

  • Expressed
  • Sequence
  • Sucrose
  • Synthase
  • TAG