Effects of catecholamines on secretion of interferon tau and expression of genes for synthesis of polyamines and apoptosis by ovine trophectoderm. Academic Article uri icon


  • Ovine trophectoderm (oTr1) cells were used to investigate effects of epinephrine (EP), norepinephrine (NE), and dopamine (DA) on their proliferation, migration and adhesion, secretion of interferon tau (IFNT), and expression of genes for synthesis of polyamines and apoptosis. Expression of mRNAs for agmatinase (AGMAT), arginine decarboxylase (ADC), ornithine decarboxylase (ODC1), and solute carrier family 7 (SLC7A1) (cationic amino acid transporter, Y+system), member 1 increased (P<0.05) in oTr1 cells in response to EP and DA. However, expression of SLC7A1 decreased at high doses of EP and expression of ADC mRNA by oTr1 cells decreased in response to 20 and 40ng/ml NE, and 40ng/ml DA. Migration of oTr1 cells increased in response to EP, DA, and NE after 48 h of treatment. However, proliferation of oTr1 cells was inhibited by 300pg/ml EP after 96h and DA at 20 and 100ng/ml. EP increased adhesion of oTr1 cells. The secretion of IFNT increased in response to 300pg/ml EP, 100ng/ml NE and DA after 48 h and at 96 h, and both DA (40ng/ml) and NE (100ng/ml). Expression of mRNAs for apoptotic genes (caspase 3, cathpsin B, BCL2 associated X protein "bax," B-cell lymphoma 2 "bcl2," and proto-oncogene "cmyc") decreased (P<0.05) in response to catecholamines, but DA did not affect (P<0.05) expression of cMYC mRNA. These results indicate that catecholamines play important roles in conceptus development during the peri-implantation period of pregnancy through effects on synthesis of polyamines, secretion of IFNT, and expression of apoptotic genes by oTr1 cells.

published proceedings

  • Biol Reprod

altmetric score

  • 0.75

author list (cited authors)

  • Elmetwally, M. A., Lenis, Y., Tang, W., Wu, G., & Bazer, F. W.

citation count

  • 15

complete list of authors

  • Elmetwally, Mohammed A||Lenis, Yasser||Tang, Wanjin||Wu, Guoyao||Bazer, Fuller W

publication date

  • September 2018