Aspergillus nidulans polarity mutant swoA is complemented by protein O-mannosyltransferase pmtA. Academic Article

abstract

• Previously swoA was identified in Aspergillus nidulans as a single locus, temperature-sensitive (ts) mutant aberrant in polarity maintenance. swoA was complemented by transformation with a plasmid genomic library. The sequence of the complementing gene was identical to a previously submitted GenBank sequence for pmtA, a protein O-mannosyltransferase. The pmtA/swoA-2 gene hybridized to three cosmids that are located on chromosome V and the swoA mutation was mitotically mapped to chromosome V. PMTs are endoplasmic reticulum-resident proteins responsible for the first step of O-glycosylation. Structural predictions suggest that PmtA contains seven membrane spans similar to PMTs from Saccharomyces cerevisiae and other organisms. Phylogenetic analysis indicates that PmtA is most closely related to the S. cerevisiae sub-family of PMTs containing Pmt2, Pmt3 and Pmt6. The mutant pmtA/swoA-2 locus contained a lesion that changed Y662 to a stop codon, eliminating the final membrane spanning domain and interrupting a domain essential for function in other PMTs.

authors

• Shaw, Brian

published proceedings

• Fungal Genet Biol

author list (cited authors)

• Shaw, B. D., & Momany, M.

citation count

• 49

complete list of authors

• Shaw, Brian D||Momany, Michelle

publication date

• January 2002

publisher

• Elsevier  Publisher

published in

• n1087-1845ISSN  Journal

keywords

• Amino Acid Sequence
• Aspergillus Nidulans
• Cell Polarity
• Chromosome Mapping
• Fungal Proteins
• Genetic Complementation Test
• Glycosylation
• Mannosyltransferases
• Methyltransferases
• Molecular Sequence Data
• Mutation
• Phylogeny
• Sequence Alignment
• Sequence Analysis, DNA

Digital Object Identifier (DOI)

• 10.1016/s1087-1845(02)00531-5

start page

• 263

end page

• 270

volume

• 37

issue

• 3

URL

• http://dx.doi.org/10.1016/s1087-1845(02)00531-5