The HopPtoF locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector. Academic Article uri icon

abstract

  • Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcF(Pto) and HopF(Pto), that are preceded by a single hrp box promoter. We present evidence here to show that ShcF(Pto) and HopF(Pto) encode a type III chaperone and a cognate effector, respectively. ShcF(Pto) interacts with and stabilizes the HopF(Pto) protein in the bacterial cell. Translation of HopF(Pto) starts at a rare initiation codon ATA that limits the synthesis of the HopF(Pto) protein to a low level in bacterial cells.

published proceedings

  • Mol Plant Microbe Interact

altmetric score

  • 1

author list (cited authors)

  • Shan, L., Oh, H., Chen, J., Guo, M., Zhou, J., Alfano, J. R., ... Tang, X.

citation count

  • 35

complete list of authors

  • Shan, Libo||Oh, Hye-sook||Chen, Jianfu||Guo, Ming||Zhou, Jianmin||Alfano, James R||Collmer, Alan||Jia, Xu||Tang, Xiaoyan

publication date

  • May 2004