A translational enhancer element on the 3'-proximal end of the Panicum mosaic virus genome. Academic Article

abstract

• Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3'-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5'-proximal sgRNA genes in vitro.

authors

• Scholthof, Karen-Beth

published proceedings

• FEBS Lett

author list (cited authors)

• Batten, J. S., Desvoyes, B., Yamamura, Y., & Scholthof, K.

citation count

• 42

complete list of authors

• Batten, Jeffrey S||Desvoyes, Benedicte||Yamamura, Yoshimi||Scholthof, Karen-Beth G

publication date

• May 2006

publisher

• Wiley  Publisher

published in

• FEBS Letters  Journal

keywords

• 3' Untranslated Regions
• Base Sequence
• Genome, Viral
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• Protein Biosynthesis
• Regulatory Sequences, Ribonucleic Acid
• Tombusviridae

PubMed Central ID

• 16647707

Digital Object Identifier (DOI)

• 10.1016/j.febslet.2006.04.006

start page

• 2591

end page

• 2597

volume

• 580

issue

• 11

URL

• http://dx.doi.org/10.1016/j.febslet.2006.04.006