RNA: protein interactions associated with satellites of panicum mosaic virus. Academic Article

abstract

• The interactions between satellite panicum mosaic virus (SPMV) capsid protein (CP) and its 824 nucleotide (nt) single stranded RNA were investigated by gel mobility shift assay and Northwestern blot assay. SPMV CP has specificity for its RNA at high affinity, but little affinity for non-viral RNA. The SPMV CP also bound a 350 nt satellite RNA (satRNA) that, like SPMV, is dependent on panicum mosaic virus for its replication. SPMV CP has the novel property of encapsidating SPMV RNA and satRNA. Competition gel mobility shift assays performed with a non-viral RNA and unlabeled SPMV RNA and satRNA revealed that these RNA:protein interactions were in part sequence specific.

authors

• Scholthof, Karen-Beth

published proceedings

• FEBS Lett

author list (cited authors)

• Desvoyes, B., & Scholthof, K. B.

citation count

• 24

complete list of authors

• Desvoyes, B||Scholthof, KB

publication date

• November 2000

publisher

• Wiley  Publisher

published in

• FEBS Letters  Journal

keywords

• Capsid
• Centrifugation, Density Gradient
• Mosaic Viruses
• Panicum
• RNA, Viral
• Satellite Viruses

PubMed Central ID

• 11086159

Digital Object Identifier (DOI)

• 10.1016/s0014-5793(00)02177-3

start page

• 25

end page

• 28

volume

• 485

issue

• 1

URL

• http://dx.doi.org/10.1016/s0014-5793(00)02177-3