Integration of satellite bacteriophage P4 in Escherichia coli. DNA sequences of the phage and host regions involved in site-specific recombination. Academic Article

abstract

• We determined the DNA sequences of regions essential for bacteriophage P4 integration. A 20 base-pair core sequence in both phage (P4attP) and host (P4attB) attachment regions contains the recombination site. In P4attP this sequence is flanked by five repeated sequences. A 1.3 x 10(3) base open reading frame codes for P4 integrase. Two possible promoters are upstream from P4int. One would be recognized by Escherichia coli RNA polymerase and may be repressed by integrase protein. The second would be recognized by RNA polymerase modified after infection by a P4 helper phage, P2. The P4attB core sequence is the 3' end of a leucine tRNA gene. Downstream from this tRNA in E. coli K-12 is a region homologous to P4int that may be part of a cryptic prophage.

authors

• Pierson, Leland

published proceedings

• J Mol Biol

author list (cited authors)

• Pierson, L. S., & Kahn, M. L.

citation count

• 101

complete list of authors

• Pierson, LS||Kahn, ML

publication date

• January 1987

publisher

• Elsevier  Publisher

published in

• Journal of Molecular Biology  Journal

keywords

• Bacteriophages
• Base Sequence
• Beta-Galactosidase
• DNA, Recombinant
• DNA, Viral
• Dna Nucleotidyltransferases
• Escherichia Coli
• Integrases
• Lysogeny
• Molecular Sequence Data
• Plasmids
• Promoter Regions, Genetic
• Sequence Homology, Nucleic Acid

Digital Object Identifier (DOI)

• 10.1016/0022-2836(87)90026-x

start page

• 487

end page

• 496

volume

• 196

issue

• 3

URL

• http://dx.doi.org/10.1016/0022-2836(87)90026-x