DNABased Markers for a Recessive Gene Conferring Anthracnose Resistance in Sorghum
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Resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench] accession SC326-6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623. F3 progeny tests following self pollination of 115 F2individuals identified homozygous resistant and susceptible F2plants for use in bulked-segregant analysis. DNA from the parental cultivars and the bulks was screened by PCR amplification with 300 RAPD (random amplified polymorphic DNA) primers. Two RAPD primers amplified a sequence that co-segregated with the recessive resistance allele, while another amplified a band linked to the susceptible allele.
author list (cited authors)
Boora, K. S., Frederiksen, R., & Magill, C.
complete list of authors
Boora, Khazan S||Frederiksen, Richard||Magill, Clint