DNABased Markers for a Recessive Gene Conferring Anthracnose Resistance in Sorghum Academic Article uri icon

abstract

  • Resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench] accession SC326-6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623. F3 progeny tests following self pollination of 115 F2individuals identified homozygous resistant and susceptible F2plants for use in bulked-segregant analysis. DNA from the parental cultivars and the bulks was screened by PCR amplification with 300 RAPD (random amplified polymorphic DNA) primers. Two RAPD primers amplified a sequence that co-segregated with the recessive resistance allele, while another amplified a band linked to the susceptible allele.

published proceedings

  • Crop Science

author list (cited authors)

  • Boora, K. S., Frederiksen, R., & Magill, C.

citation count

  • 36

complete list of authors

  • Boora, Khazan S||Frederiksen, Richard||Magill, Clint

publication date

  • November 1998

publisher