The Florida manatee (Trichechus manatus latirostris) T cell receptor loci exhibit V subgroup synteny and chain-specific evolution. Academic Article uri icon

abstract

  • The Florida manatee (Trichechus manatus latirostris) has limited diversity in the immunoglobulin heavy chain. We therefore investigated the antigen receptor loci of the other arm of the adaptive immune system: the T cell receptor. Manatees are the first species from Afrotheria, a basal eutherian superorder, to have an in-depth characterization of all T cell receptor loci. By annotating the genome and expressed transcripts, we found that each chain has distinct features that correlates to their individual functions. The genomic organization also plays a role in modulating sequence conservation between species. There were extensive V subgroup synteny blocks in the TRA and TRB loci between T.m. latirostris and human. Increased genomic locus complexity correlated to increased locus synteny. We also identified evidence for a VHD pseudogene for the first time in a eutherian mammal. These findings emphasize the value of including species within this basal eutherian radiation in comparative studies.

published proceedings

  • Dev Comp Immunol

author list (cited authors)

  • Breaux, B., Hunter, M. E., Cruz-Schneider, M. P., Sena, L., Bonde, R. K., & Criscitiello, M. F.

citation count

  • 8

complete list of authors

  • Breaux, Breanna||Hunter, Margaret E||Cruz-Schneider, Maria Paula||Sena, Leonardo||Bonde, Robert K||Criscitiello, Michael F

publication date

  • January 2018