Transformation of TAES cotton for seedling disease resistance Conference Paper uri icon

abstract

  • Five TAES cotton (Gossypium hirsutum L.) cultivars were transformed using two vectors, pXBOP and pJMA4, which contained genes associated with plant pathogenesis (PR genes): osmotin (PR-5) and a basic PR-1 in pXBOP, and lox1 in pJMA4. Plants were transformed using the shoot apex/Agrobacterium method (US Pat. 5,164,310; Smith et al., 1992) developed earlier for cotton (Gould et al., 1991a; 1991b). After kanamycin selection, shoots (T0) were grafted onto in vitro grown seedling rootstocks (Luo and Gould, 1999). Grafted/regenerated plants were normal, fertile, and produced normal progeny. The transferred genes were detected in the DNA of regenerated plants (T0) and in progeny (T1) using PCR amplification and Southern DNA analyses. Gene expression was detected by RT-PCR in the progeny.

published proceedings

  • 2000 Proceedings Beltwide Cotton Conferences

author list (cited authors)

  • Luo, J., Arriaga, J. M., Li, H., Hemphill, J. K., Raisor, M., Keller, N., Isakeit, T., & Gould, J. H.

complete list of authors

  • Luo, J||Arriaga, JM||Li, H||Hemphill, JK||Raisor, M||Keller, N||Isakeit, T||Gould, JH

publication date

  • January 2000