Metabolism of Exogenous Oxaloacetate in Isolated Hepatocytes
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The utilization of exogenous oxaloacetate (OAA) was studied in isolated rat liver parenchymal cells. Phenylpyruvate and α-cyanohydroxycinnamate, which inhibit pyruvate carboxylase (EC 188.8.131.52) and pyruvate transport across the mitochondrial membrane, respectively, were equally effective in decreasing glucose formation from pyruvate and OAA. Additionally, the specific activity of glucose formed in cells incubated with sodium [14C]bicarbonate and either pyruvate or OAA was the same. The specific activity of glucose formed from asparagine plus sodium [14C]bicarbonate was approximately 25% of that observed when pyruvate or OAA served as the carbon sources. The results suggest that OAA is decarboxylated to pyruvate prior to its metabolism. © 1981, SAGE Publications. All rights reserved.
author list (cited authors)
Smith, S. B., & Freedland, R. A.