Survival of Tn5 mutant bean rhizobia in desert soils: Phenotypic expression of Tn5 under moisture stress
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The survival and phenotypic expression of a transposon Tn5 mutant of bean rhizobia (Rhizobium leguminosarum bv. phaseoli) and the corresponding wildtype under increasing moisture stress conditions was studied in two Sonoran Desert soil types. Survival studies in sterile and nonsterile soil showed that both biotic and abiotic factors influenced the survival of the mutant and the wild type. In the silty clay loam, the Tn5 mutant population averaged ca 105cfu g-1 soil even at a moisture content of 2% (corresponding to a soil water potential of