Detection of Pathogenic Bacteria in the Pork Industry Chain Using a Surface Plasmon Resonance Biosensor Part 1: Sensor Verification
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abstract
A surface plasmon resonance (SPR) biosensor is employed in this study to detect Salmonella typhimurium, Listeria monocytogenes, and Escherichia coli O157:H7, three of the deadliest foodborne pathogens in the United States. The SPR biosensor couples antibody-antigen reactions with signal generation, using the Kretschmann geometry to couple light into surface plasmons. The objective of this study was to verify the feasibility of employing an SPR biosensor to detect Salmonella spp., Listeria spp., and E. coli O157:H7 by testing the sensitivity of the SPR biosensor assays. Tests were conducted at near real-time with results obtained for one SPR biosensor assay in 29 min. The limit of detection for Salmonella spp. was determined to be 10 1 CFU/ml, 10 1 CFU/ml for Listeria spp., and 10 5 CFU/ml for E. coli O157:H7. The results of this study demonstrate the potential for employing the SPR biosensor to detect pathogenic bacteria at very low levels, in near real-time. The SPR biosensor shows potential to fulfill the meat industry need for pathogen monitoring techniques of automation, near real-time results, sensitivity, accuracy and portability, and could potentially be incorporated into a HACCP protocol for meat processing.
