Intercalibration of gas chromatographic analyses for hydrocarbons in tissues and extracts of marine organisms.
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Gas chromatographic analyses of hydrocarbons separated from tuna meal samples and cod liver lipid extracts have been intercalibrated among three laboratories. Measurement of petroleum hydrocarbons spiked to samples of cod liver oil gave values as follows: (formula omitted) distillate cut of South Louisiana crude oil372 g/g, 0.09, 0.06; No. 2 fuel oil1163 /g, 0.50, 0.26; Wilmington crude oil913 g/g, 0.69, 0.34. The estimates of petroleum hydrocarbons In tuna meal subsamples gave x s of 37.7 4.6 g/g dry weight. Measurements of pristane In cod liver lipid samples gave x s of 35.7 3.5 pg/g lipid and 271 4.5 pg/g lipid. Measurements of pristane in tuna meal were less precise with x s of 2.4 1.5 g/g dry weight. Some limitations to current methods of analysis as applied in this study and in several current oil pollution studies are demonstrated and discussed. 1976, American Chemical Society. All rights reserved.