Anti-inflammatory activity of polyphenolics from aai (Euterpe oleracea Martius) in intestinal myofibroblasts CCD-18Co cells.
Overview
Research
Identity
Additional Document Info
Other
View All
Overview
abstract
The demand for tropical fruits high in polyphenolics including aai (Euterpe oleracea Mart.) has been increasing based on ascribed health benefits and antioxidant properties. This study evaluated the anti-inflammatory activities of aai polyphenolics in human colon myofibroblastic CCD-18Co cells to investigate the suppression of reactive oxygen species (ROS), and mRNA and protein expression of inflammatory proteins. Non-cytotoxic concentrations of aai extract, 1-5 mg gallic acid equivalent L(-1), were selected. The generation of ROS was induced by lipopolysaccharide (LPS) and aai extract partially reversed this effect to 0.53-fold of the LPS-control. Aai extract (5 mg GAE L(-1)) down-regulated LPS-induced mRNA-expression of tumor necrosis factor alpha, TNF- (to 0.42-fold), cyclooxygenase 2, COX-2 (to 0.61-fold), toll-like receptor-4, TLR-4 (to 0.52-fold), TNF receptor-associated factor 6, TRAF-6 (to 0.64-fold), nuclear factor kappa-B, NF-B (to 0.76-fold), vascular cell adhesion molecule 1, VCAM-1 (to 0.71-fold) and intercellular adhesion molecule 1, ICAM-1 (to 0.68-fold). The protein levels of COX-2, TLR-4, p-NF-B and ICAM-1 were induced by LPS and the aai extract partially reversed this effect in a dose-dependent manner. These results suggest the anti-inflammatory effect of aai polyphenolic extract in intestinal cells are at least in part mediated through the inhibition of ROS and the expression of TLR-4 and NF-B. Results indicate the potential for aai polyphenolics in the prevention of intestinal inflammation.