Anti-inflammatory activity of polyphenolics from aai (Euterpe oleracea Martius) in intestinal myofibroblasts CCD-18Co cells.
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The demand for tropical fruits high in polyphenolics including aai (Euterpe oleracea Mart.) has been increasing based on ascribed health benefits and antioxidant properties. This study evaluated the anti-inflammatory activities of aai polyphenolics in human colon myofibroblastic CCD-18Co cells to investigate the suppression of reactive oxygen species (ROS), and mRNA and protein expression of inflammatory proteins. Non-cytotoxic concentrations of aai extract, 1-5 mg gallic acid equivalent L(-1), were selected. The generation of ROS was induced by lipopolysaccharide (LPS) and aai extract partially reversed this effect to 0.53-fold of the LPS-control. Aai extract (5 mg GAE L(-1)) down-regulated LPS-induced mRNA-expression of tumor necrosis factor alpha, TNF- (to 0.42-fold), cyclooxygenase 2, COX-2 (to 0.61-fold), toll-like receptor-4, TLR-4 (to 0.52-fold), TNF receptor-associated factor 6, TRAF-6 (to 0.64-fold), nuclear factor kappa-B, NF-B (to 0.76-fold), vascular cell adhesion molecule 1, VCAM-1 (to 0.71-fold) and intercellular adhesion molecule 1, ICAM-1 (to 0.68-fold). The protein levels of COX-2, TLR-4, p-NF-B and ICAM-1 were induced by LPS and the aai extract partially reversed this effect in a dose-dependent manner. These results suggest the anti-inflammatory effect of aai polyphenolic extract in intestinal cells are at least in part mediated through the inhibition of ROS and the expression of TLR-4 and NF-B. Results indicate the potential for aai polyphenolics in the prevention of intestinal inflammation.
author list (cited authors)
Dias, M., Martino, H., Noratto, G., Roque-Andrade, A., Stringheta, P. C., Talcott, S., Ramos, A. M., & Mertens-Talcott, S. U.
complete list of authors
Dias, Manoela Maciel dos Santos||Martino, Hércia Stampini Duarte||Noratto, Giuliana||Roque-Andrade, Andrea||Stringheta, Paulo César||Talcott, Stephen||Ramos, Afonso Mota||Mertens-Talcott, Susanne U