Rubisco activities and the small subunit gene cloning and functional analysis of Aegilops squarrosa

The plant materials used were the leaves of Aegilops squarrosa L. , Triticum aestivum L. and a nucleo-cytoplasmic hybrid wheat which was obtained by crossing T. aestivum( )with A. squarrosa ( )and backcrossing 10 generations with T. aestivum as the paternal parent. The car-boxylase and oxygenase activities of these materials were assayed. At the same time, a gt10 cDNA library of 5. 5 105pfu was constructed with poly (A) RNA from the leaves of A. squarrosa seedlings. This library was screened with a partial fragment of rice rbcS, and a cDNA clone pRAS-1 that includes A. squarrosa rbcS was obtained. Nucleotide sequencing indicated that the full length of this cDNA was 815 bp. Comparison of small subunit of ribulose-1,5-bisphosphate carboxylase/ oxygenase (rubisco) from A. squarrosa with that from T. aestivum and their nucleo-cytoplasmic hybrid wheat revealed that the amino acid residues at positions 56, 84, 98 and 117 in the small subunit might play important roles in regulating the activities of rubisco.

Rubisco activities and the small subunit gene cloning and functional analysis of Aegilops squarrosa Academic Article