Effect of temperature on sperm quality of captive Litopenaeus vannamei broodstock Academic Article uri icon


  • Effect of temperature on reproductive quality of captive male Litopenaeus vannamei broodstock was investigated by measuring sperm count and percentage of abnormal sperm per compound spermatophore. Variability in these responses among and within experimental units also was evaluated. Male shrimp with an initial average weight of 48.0 g were maintained in 3.7-m diameter circular tanks with recirculating seawater, and exposed to constant temperatures of 26C, 29C, and 32C for 42 days. Significantly higher mean sperm count (18.6 million cells) and lower percentage of sperm abnormalities (36.7%) were observed in shrimp held at 26C, as compared to broodstock at 29C (mean sperm count = 0.1 million cells; percentage of sperm abnormalities = 99.7%) or 32C (no sperm cells) (P < 0.05). Results indicate that adequate sperm count and percentage of abnormal sperm of L. vannamei broodstock can be maintained at water temperature of 26C, but not at 29C or 32C, at least within the time span of 42 days. The importance of treatment replication and sampling sufficient numbers of shrimp from culture tanks in experiments concerning male reproductive quality is discussed. 2001 Elsevier Science B.V.

published proceedings

  • Aquaculture

author list (cited authors)

  • Perez-Velazquez, M., Bray, W. A., Lawrence, A. L., Gatlin, D. M., & Gonzalez-Felix, M. L.

citation count

  • 44

complete list of authors

  • Perez-Velazquez, Martin||Bray, William A||Lawrence, Addison L||Gatlin, Delbert M||Gonzalez-Felix, Mayra L

publication date

  • July 2001