A single cell multi-analysis system for electrophysiological studies Conference Paper uri icon


  • 2003 IEEE. A micro analysis system for multi-purpose electrophysiological studies of single cells has been designed, fabricated, and characterized. The micro electrophysiological analysis system (-EPAS) contains multiple extracellular opposing electrode pairs that interface with a single cell inside an analysis cavity. These electrodes can be used for general single electrode extracellular stimulation/recording studies, dual electrode applications such as impedance spectroscopy, and for applications requiring a greater number of electrodes such as impedance tomography. Additionally, the analysis system contains a sealing/clamping mechanism and a backside extracellular reference electrode for patch clamping studies. The analysis cavities were designed in an array format for parallel analyses. Bovine chromaffin cells were used as a target to demonstrate and characterize the system. Using the integrated glass sealing ring, giga-seals were obtained on a population of chromaffin cells. The micro system generated maximum giga-seal resistances of 1.2G with non-sealed average cavity-to-backside resistances of 0.5M . Furthermore, the cellular analysis system was demonstrated for electrical impedance spectroscopy on chromaffin cells over a range from 40.0 Hz to 1.0 MHz.

name of conference

  • TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664)

published proceedings


altmetric score

  • 3

author list (cited authors)

  • Han, A., Moss, E., Rabbitt, R. D., Engisch, K. L., & Frazier, A. B.

citation count

  • 7

complete list of authors

  • Han, A||Moss, E||Rabbitt, RD||Engisch, KL||Frazier, AB

publication date

  • January 2003