MARKER-ASSISTED SELECTION FOR PVY RESISTANCE IN TETRAPLOID POTATOES
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Potato virus Y (PVY, Potyvirus) is the most harmful disease in cultivated potatoes, affecting tuber seed production, tuber yield and quality. The most common method to limit PVY is to plant certified potato seed with low virus levels. However, milder foliar symptoms caused by some of the PVY strains, or no foliar symptoms in some asymptomatic potato cultivars makes visual detection of PVY difficult and increases the levels of PVY in certified seed. In addition, insecticides are ineffective to control PVY due to the non-persistent transmission of the virus by the aphids. The use of host plant resistance provides a cost-effective and environmentally friendly alternative. Extreme non-strain specific PVY resistant genes derived from S. tuberosum ssp. andigena (Ryadg gene) and from S. stoloniferum (Rysto gene) have been introgressed into tetraploid Tri-State potato clones. Genetic mapping efforts located the extreme resistant Ryadg gene on Chr. XI and the Rysto gene on Chr. XII and PCR-based markers linked to those genes have been previously developed. We have validated the presence and transmission of the Ryadg and Rysto genes in our clones and applied marker-assisted selection (MAS) in our tetraploid breeding program to expedite the development of PVY resistant potato cultivars.
