Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5. Academic Article uri icon

abstract

  • Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.

published proceedings

  • Nat Biotechnol

altmetric score

  • 24

author list (cited authors)

  • Paulsen, I. T., Press, C. M., Ravel, J., Kobayashi, D. Y., Myers, G., Mavrodi, D. V., ... Loper, J. E.

citation count

  • 529

complete list of authors

  • Paulsen, Ian T||Press, Caroline M||Ravel, Jacques||Kobayashi, Donald Y||Myers, Garry SA||Mavrodi, Dmitri V||DeBoy, Robert T||Seshadri, Rekha||Ren, Qinghu||Madupu, Ramana||Dodson, Robert J||Durkin, A Scott||Brinkac, Lauren M||Daugherty, Sean C||Sullivan, Stephen A||Rosovitz, Mary J||Gwinn, Michelle L||Zhou, Liwei||Schneider, Davd J||Cartinhour, Samuel W||Nelson, William C||Weidman, Janice||Watkins, Kisha||Tran, Kevin||Khouri, Hoda||Pierson, Elizabeth A||Pierson, Leland S||Thomashow, Linda S||Loper, Joyce E

publication date

  • January 2005