An Autocrine Proliferation Repressor Regulates Dictyostelium discoideum Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH. Academic Article uri icon


  • In eukaryotic microbes, little is known about signals that inhibit the proliferation of the cells that secrete the signal, and little is known about signals (chemorepellents) that cause cells to move away from the source of the signal. Autocrine proliferation repressor protein A (AprA) is a protein secreted by the eukaryotic microbe Dictyostelium discoideum AprA is a chemorepellent for and inhibits the proliferation of D.discoideum We previously found that cells sense AprA using G proteins, suggesting the existence of a G protein-coupled AprA receptor. To identify the AprA receptor, we screened mutants lacking putative G protein-coupled receptors. We found that, compared to the wild-type strain, cells lacking putative receptor GrlH (grlH cells) show rapid proliferation, do not have large numbers of cells moving away from the edges of colonies, are insensitive to AprA-induced proliferation inhibition and chemorepulsion, and have decreased AprA binding. Expression of GrlH in grlH cells (grlH/grlHOE ) rescues the phenotypes described above. These data indicate that AprA signaling may be mediated by GrlH in D.discoideumIMPORTANCE Little is known about how eukaryotic cells can count themselves and thus regulate the size of a tissue or density of cells. In addition, little is known about how eukaryotic cells can sense a repellant signal and move away from the source of the repellant, for instance, to organize the movement of cells in a developing embryo or to move immune cells out of a tissue. In this study, we found that a eukaryotic microbe uses G protein-coupled receptors to mediate both cell density sensing and chemorepulsion.

published proceedings

  • mBio

altmetric score

  • 2

author list (cited authors)

  • Tang, Y. u., Wu, Y., Herlihy, S. E., Brito-Aleman, F. J., Ting, J. H., Janetopoulos, C., & Gomer, R. H.

citation count

  • 10

complete list of authors

  • Tang, Yu||Wu, Yuantai||Herlihy, Sarah E||Brito-Aleman, Francisco J||Ting, Jose H||Janetopoulos, Chris||Gomer, Richard H

editor list (cited editors)

  • Emr, S. D.

publication date

  • February 2018

published in