High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples. Academic Article uri icon

abstract

  • We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost.

published proceedings

  • J Microbiol Methods

author list (cited authors)

  • Brady, J. A., Faske, J. B., Castaeda-Gill, J. M., King, J. L., & Mitchell, F. L.

citation count

  • 8

complete list of authors

  • Brady, Jeff A||Faske, Jennifer B||CastaƱeda-Gill, Jessica M||King, Jonathan L||Mitchell, Forrest L

publication date

  • September 2011