High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples. Academic Article

abstract

• We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost.

authors

• Mitchell, Forrest

published proceedings

• J Microbiol Methods

author list (cited authors)

• Brady, J. A., Faske, J. B., Castaeda-Gill, J. M., King, J. L., & Mitchell, F. L.

citation count

• 8

complete list of authors

• Brady, Jeff A||Faske, Jennifer B||Castañeda-Gill, Jessica M||King, Jonathan L||Mitchell, Forrest L

publication date

• January 2011

publisher

• Elsevier  Publisher

published in

• Journal of Microbiological Methods  Journal

keywords

• Animals
• DNA, Bacterial
• High-Throughput Screening Assays
• INSECTA
• Plant Diseases
• Plants
• Xylella

PubMed Central ID

• 21703312

Digital Object Identifier (DOI)

• 10.1016/j.mimet.2011.06.007

start page

• 310

end page

• 312

volume

• 86

issue

• 3

URL

• http://dx.doi.org/10.1016/j.mimet.2011.06.007