Identification and characterization of 10 polymorphic microsatellite loci in the German cockroach, Blattella germanica Academic Article uri icon

abstract

  • Primer sequences and initial characterization are presented for 10 microsatellite loci isolated from the German cockroach, Blattella germanica. In a sample of 30 individuals from a single population sample, all loci were polymorphic with two to 12 alleles segregating per locus and levels of observed heterozygosity ranging from 0.27 to 0.92. One locus showed a deficit of heterozygotes. Experimental conditions are described for polymerase chain reaction multiplexing, which enables the genotyping of eight loci in three electrophoretic runs consisting of one set of three and two sets of two markers. Seven primer sets cross-amplify in the related Blattella asahinai. 2006 The Authors.

published proceedings

  • MOLECULAR ECOLOGY NOTES

author list (cited authors)

  • Booth, W., Bogdanowicz, S. M., Prodohl, P. A., Harrison, R. G., Schal, C., & Vargo, E. L.

citation count

  • 11

complete list of authors

  • Booth, W||Bogdanowicz, SM||Prodohl, PA||Harrison, RG||Schal, C||Vargo, EL

publication date

  • January 1, 2007 11:11 AM

publisher