Genome size determination using flow cytometry of propidium iodide-stained nuclei. Academic Article

abstract

• With the rapid expansion of whole-genome sequencing and other genomic studies in nonmodel -organisms, there is a growing demand for robust and user-friendly methods for estimating eukaryotic genome sizes across a broad range of taxa. Propidium iodide (PI) staining with flow cytometry is a powerful method for genome sizing because it is relatively fast, works with a wide variety of materials, and provides information on a very large number of nuclei. In this method, nuclei are stained with PI, which intercalates into the major groove of DNA. Unknown samples are typically costained with standard nuclei of a known genome size, and the relative fluorescence is used to calculate the genome size of the unknown.

authors

• Johnston, J

published proceedings

• Methods Mol Biol

author list (cited authors)

• Hare, E. E., & Johnston, J. S.

citation count

• 103

complete list of authors

• Hare, Emily E||Johnston, J Spencer

publication date

• January 2011

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Cell Nucleus
• Chickens
• Drosophila Melanogaster
• Female
• Flow Cytometry
• Frozen Sections
• Genome Size
• Houseflies
• Propidium
• Staining And Labeling
• Statistics As Topic

Digital Object Identifier (DOI)

• 10.1007/978-1-61779-228-1_1

start page

• 3

end page

• 12

volume

• 772

URL

• http://dx.doi.org/10.1007/978-1-61779-228-1_1