Determination of amphetamines and ketamine in biological specimens by liquid chromatography - Tandem mass spectrometry
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Objective: To develop a method for simultaneous determination of amphetamines and ketamine in biological specimens by liquid chromatography - tandem mass spectrometry (LC - MS/MS). Methods: The parent drugs and their metabolites were isolated simultaneously from human plasma, urine and hair by dilution and LLE. The parameters of LC - MS/MS including two columns were optimized. Ion suppression effects of matrixes were reviewed. Results: Amphetamine and MDA were detected simultaneously with Liquid phase 1 ran within 3 min. The compounds were isolated from matrix and confirmed with Liquid phase 2. The amount of sample was less required by urine dilution method that was prepared simply and rapidly. Limits of detection ranged from 0.005 ng/mg to 0.05ng/mg. We also analyzed the hair segments from a postpartum woman and her infant. Conclusion: The method developed may be applied for simultaneous determination of amphetamines and ketamine in biological specimens with small quantities of samples. The matrix effect of plasma and urine is the primary factor influencing the sensitivity.
Chinese Journal of Forensic Medicine
author list (cited authors)
Xiang, P., Zhuo, X. Y., & Shen, B. H.
complete list of authors
Xiang, P||Zhuo, XY||Shen, BH