Two new species of anchialine cave shrimp from Cozumel Island are described. Anew species from the family Agostocarididae,
Agostocaris zabaletain. sp., occurs in three cenotes on the island (Chempita, Chankanaab System and Tres Potrillos). This new species is closely related to Agostocaris bozanicithat is known from one isolated cenote in another zone of the island. Agostocaris zabaletain. sp. is similar to A. bozanicibecause it lacks a rostrum with teeth, and the palp of the first maxilliped is not segmented. In addition A. zabaletaialso differs from A. bozaniciby bearing four pairs of spines on the telson and only one spine on the outer ramus of the uropods. Both Agostocaris williamsiand Agostocaris acklinsensisdiffer from A. zabaletaibecause both species have occasionally a serrate rostrum, and these species are reported exclusively from the Bahamas. Anew family, Anchialocarididae, new genus and species Anchialocaris paulinin. sp. are described. This new species is known exclusively from the cenote Chempita located in the central area of the island. The new family Anchialocarididae is closely related to the families Agostocarididae and Atyidae because they have a similar branchial formulae. The main difference is that the new family has a simple articulation between carpus and palm of the first pereiopod, and this appendage is more slender than the second. Although the new family has several setae on the rudimentary propodus on the second pereiopod it lacks the abundance of setae found in other members of the Atyidae family genus Typhlatya. The relationship between this new family and the Agostocarididae is discussed.