Accurate titration of avidin and streptavidin with biotin-fluorophore conjugates in complex, colored biofluids. Academic Article

abstract

• A new fluorimetric assay is presented for the specific and reliable quantitation of >/=2 nM avidin and streptavidin. The assay is based on pronounced changes in the fluorescence properties of commercial fluorescein-biotin, or of a newly synthesized biotin-poly(ethylene glycol)-pyrene conjugate, which occur upon binding to avidin and streptavidin. Accurate measurement of (strept)avidin in complex, colored biofluids, such as crude egg white or serum relies on a simple titration protocol. Only occasional recalibration of the reagent solution is required. Due to these merits the proposed assay is particularly suited for rapid measurement of few samples on short notice, for functional control of (strept)avidin-containing reagents after storage, and for the monitoring of (strept)avidin concentrations in large scale processes.

authors

• Kaiser, Karl

published proceedings

• Biochim Biophys Acta

altmetric score

• 3

author list (cited authors)

• Gruber, H. J., Kada, G., Marek, M., & Kaiser, K.

citation count

• 50

complete list of authors

• Gruber, HJ||Kada, G||Marek, M||Kaiser, K

publication date

• January 1998

publisher

• Elsevier  Publisher

published in

• Biochimica et Biophysica Acta: international journal of biochemistry and biophysics  Journal

keywords

• Animals
• Avidin
• Binding Sites
• Biotin
• Color
• Fluorescent Dyes
• Indicators And Reagents
• Reference Standards
• Spectrometry, Fluorescence
• Streptavidin

Digital Object Identifier (DOI)

• 10.1016/s0304-4165(98)00029-4

start page

• 203

end page

• 212

volume

• 1381

issue

• 2

URL

• http://dx.doi.org/10.1016/s0304-4165(98)00029-4