Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene. Chapter

abstract

• In Escherichia coli, conventional amber and ochre stop codons can be separately targeted by engineered amber-suppressing Methanocaldococcus jannaschii tyrosyl-tRNA synthetase-tRNAPyl and ochre-suppressing Methanosarcina maezi pyrrolysyl-tRNA synthetase-tRNAPyl pairs for coding two different noncanonical amino acids in one protein gene. Here, we describe the application of this approach to produce a protein with two distinct chemical functionalites which can be selectively labeled with two fluorescent dyes.

authors

• Liu, Wenshe

author list (cited authors)

• Tharp, J. M., & Liu, W. R.

citation count

• 6

complete list of authors

• Tharp, Jeffery M||Liu, Wenshe R

Book Title

• NONCANONICAL AMINO ACIDS: METHODS AND PROTOCOLS

publication date

• January 2018

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Amber Codon
• Amino Acids
• Codon, Nonsense
• Codon, Terminator
• Double Incorporation
• Escherichia Coli
• Gene Expression
• Mjtyrrs
• Noncanonical Amino Acid
• Ochre Codon
• Plasmids
• Protein Biosynthesis
• Protein Engineering
• Protein Labeling
• Proteins
• Pylrs
• Staining And Labeling
• Transfection

PubMed Central ID

• 29404996

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-7574-7_9

International Standard Book Number (ISBN) 13

• 978-1-4939-7573-0

start page

• 147

end page

• 154

volume

• 1728

URL

• http://dx.doi.org/10.1007/978-1-4939-7574-7_9