Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene Chapter uri icon


  • In Escherichia coli, conventional amber and ochre stop codons can be separately targeted by engineered amber-suppressing Methanocaldococcus jannaschii tyrosyl-tRNA synthetase-tRNAPyl and ochre-suppressing Methanosarcina maezi pyrrolysyl-tRNA synthetase-tRNAPyl pairs for coding two different noncanonical amino acids in one protein gene. Here, we describe the application of this approach to produce a protein with two distinct chemical functionalites which can be selectively labeled with two fluorescent dyes.

author list (cited authors)

  • Tharp, J. M., & Liu, W. R.

citation count

  • 4

complete list of authors

  • Tharp, Jeffery M||Liu, Wenshe R

Book Title

  • Noncanonical Amino Acids

publication date

  • February 2018