The effects of anhydrous ammonia on membrane stability ofP.hymatotrichum omnivorum Academic Article uri icon

abstract

  • Sclerotia and mycelium of Phymatotrichum omnivorum were exposed to anhydrous ammonia (NH3) and then observed with an electron microscope in order to determine the effects of the NH3 treatment on the fungal membranes. Sclerotia were exposed to four rates of NH3: 28, 56, 84, and 112 g NH3/ml of air for 24 hours. At 28 g/ml, the plasmalemma became wavy and the mitochondrial cristae began to swell and disperse. At 56 g NH3/ml the plasmalemma showed breakage and formation of vesicles, and all other membrane systems within the cell were broken and distorted. All membranes were totally disrupted and no organelles were recognizable at 84 g NH3/ ml. Mycelium was exposed to 2, 4, 8, 20, and 40 g NH3/ ml for one minute. Damage to cell membranes was not observed at NH3 cone. up to 4 g/ ml. At 8 g NH3/ ml the plasmalemma was broken and the mitochondria were disrupted. At 20 g/ ml and above, all internal organization was destroyed. 1982 Dr. W. Junk Publishers.

published proceedings

  • Mycopathologia

author list (cited authors)

  • Rush, C. M., & Lyda, S. D.

citation count

  • 6

complete list of authors

  • Rush, CM||Lyda, SD

publication date

  • September 1982