Identification and Mapping of Adult Plant Stripe Rust Resistance in Soft Red Winter Wheat VA00W-38 Academic Article uri icon

abstract

  • ABSTRACTThe Virginia Tech experimental wheat (Triticum aestivum L.) line VA00W38 has adult plant resistance to race PST100, which was the predominant race of stripe rust (Puccinia striiformis f. sp. tritici) in the United States from 2003 to 2006. Resistance was characterized in a cross between VA00W38 and Pioneer Brand 26R46. The mapping population consisted of 182 recombinant inbred lines, which were evaluated in field tests for stripe rust infection type (IT) in Georgia in 2008 and 2009 and for IT and severity in North Carolina in 2009 and 2010. A genomewide molecular screen with 143 simple sequence repeat and 405 diversity arrays technology markers was used to identify quantitative trait loci (QTL) for stripe rust resistance. QTL on chromosomes 2AS and 4BL of VA00W38 were identified; they explained 58.9 and 19.3% of the variation for IT and 51.9 and 12.1% of the variation for severity, respectively. QTL on chromosomes 6BL and 3BL of Pioneer 26R46 were identified and explained 8.9 and 2.1% of the variation for IT and 11.7 and 3.9% of the variation for severity. Markers Xgwm296b, Xbarc163, and Xwmc756 were most tightly linked to QTL on chromosomes 2AS, 4BL, and 6BL. Use of these markers and development of additional diagnostic markers will facilitate the incorporation and pyramiding of stripe rust resistance into soft red winter wheat lines via markerassisted selection.

published proceedings

  • CROP SCIENCE

author list (cited authors)

  • Christopher, M. D., Liu, S., Hall, M. D., Marshall, D. S., Fountain, M. O., Johnson, J. W., ... Griffey, C. A.

citation count

  • 17

complete list of authors

  • Christopher, Mark D||Liu, Shuyu||Hall, Marla D||Marshall, David S||Fountain, Myron O||Johnson, Jerry W||Milus, Eugene A||Garland-Campbell, Kimberly A||Chen, Xianming||Griffey, Carl A

publication date

  • May 2013

publisher