Mapping Net Blotch Resistance in 'Nomini' and CIho 2291 Barley
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ABSTRACTNet blotch (Pyrenophora teres) is one of the most devastating diseases of barley (Hordeum vulgare L.) worldwide. Identification of diagnostic molecular markers associated with genes and quantitative trait loci (QTL) for net blotch resistance will facilitate pyramiding of independent genes. Linkage mapping was used to identify chromosomal locations of the independent, dominant genes conditioning net blotch resistance in the winter barley Nomini (PI 566929) and spring barley CIho 2291. The F2 populations of 238 and 193 individuals, derived from crosses between the susceptible spring barley parent Hector (CIho 15514) and the resistant parents Nomini and CIho 2291, respectively, were used to map the genes governing resistance in the resistant parents. The dominant gene governing resistance in Nomini, temporarily designated RptNomini, was mapped to a 9.2cM region of barley chromosome 6H between the flanking microsatellite markers Bmag0344a (r2 = 0.7) and Bmag0103a (r2 = 0.9), which were 6.8 and 2.4 cM away from RptNomini, respectively. The dominant gene governing resistance in CIho 2291, temporarily designated RptCIho2291, was mapped to a 34.3cM interval on the distal region of barley chromosome 6H between the flanking microsatellite markers Bmag0173 (r2 = 0.65) and Bmag0500 (r2 = 0.26), which were 9.9 and 24.4 cM away from RptCIho2291, respectively. Identification of the chromosomal location of RptNomini and RptCIho2291 will facilitate efforts in pyramiding multiple genes for net blotch resistance.
