Membrane-protein binding measured with solution-phase plasmonic nanocube sensors. Academic Article uri icon


  • We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.

published proceedings

  • Nat Methods

altmetric score

  • 4.5

author list (cited authors)

  • Wu, H., Henzie, J., Lin, W., Rhodes, C., Li, Z., Sartorel, E., ... Groves, J. T.

citation count

  • 85

complete list of authors

  • Wu, Hung-Jen||Henzie, Joel||Lin, Wan-Chen||Rhodes, Christopher||Li, Zhu||Sartorel, Elodie||Thorner, Jeremy||Yang, Peidong||Groves, Jay T

publication date

  • January 2012