Membrane-protein binding measured with solution-phase plasmonic nanocube sensors. Academic Article

abstract

• We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.

authors

• Wu, Hung-Jen

published proceedings

• Nat Methods

altmetric score

• 4.5

author list (cited authors)

• Wu, H., Henzie, J., Lin, W., Rhodes, C., Li, Z., Sartorel, E., ... Groves, J. T.

citation count

• 85

complete list of authors

• Wu, Hung-Jen||Henzie, Joel||Lin, Wan-Chen||Rhodes, Christopher||Li, Zhu||Sartorel, Elodie||Thorner, Jeremy||Yang, Peidong||Groves, Jay T

publication date

• January 2012

publisher

• Springer Nature  Publisher

published in

• Nature Methods  Journal

keywords

• Calibration
• Lipid Bilayers
• Membrane Proteins
• Metal Nanoparticles
• Nanotechnology
• Protein Binding
• Silicon Dioxide
• Silver
• Solutions
• Spectrometry, Fluorescence
• Spectrophotometry, Ultraviolet
• Surface Plasmon Resonance

Digital Object Identifier (DOI)

• 10.1038/nmeth.2211

start page

• 1189

end page

• 1191

volume

• 9

issue

• 12

URL

• http://dx.doi.org/10.1038/nmeth.2211