Dynamic model of MAb production and glycosylation for the purpose of product quality control Academic Article uri icon


  • A combined model of MAb-producing mammalian cell cultures describing cell population kinetics, cell metabolism, and MAb synthesis and secretion has been developed based on previous modelling attempts from the relevant literature (Jang and Barford, 2000; Tatiraju et al., 1999; Bibila, 1991) and is in good agreement with experimental data. This is linked to a modified version of the structured compartmental model of protein glycosylation originally proposed by Umaa and Bailey (1997), which is based on a Michaelis-Menten representation of the oligosaccharide addition/removal reaction network. The dynamic results of the latter model are presented here for the first time. This coupling allows us to examine in silico and a priori the effect of any feeding strategy on the extent of MAb glycosylation and, therefore, on product quality. Preliminary optimisation results for different feeding strategies are also presented. 2005 Elsevier B.V. All rights reserved.

published proceedings

  • European Symposium on Computer-Aided Process Engineering-15, 20A and 20B

author list (cited authors)

  • Kontoravdi, C., Asprey, S. P., Pistikopoulos, S., & Mantalaris, A.

citation count

  • 1

complete list of authors

  • Kontoravdi, C||Asprey, SP||Pistikopoulos, S||Mantalaris, A

publication date

  • January 2005