Dynamic profiling of hepatocyte stress responses in a microfluidic multi-clone living cell array Conference Paper uri icon

abstract

  • Hepatocytes in the liver utilize multiple signalling pathways to integrate extracellular stimuli and orchestrate appropriate cellular responses. Conventional experimental techniques are not well suited for studying dynamics or multiple pathways in parallel. Therefore, we have constructed a experimental platform for monitoring hepatocyte inflammatory signalling non-destructively in a multi-clone living cell reporter array. The microfluidic array allows seeding, culture, stimulation, and monitoring of key signaling pathways in the hepatocyte stress response using several stable GFP reporter clones. This integrated platform will allow us to decompose the effects of individual extracellular stimuli including cytokines, growth factors, hormones, and therpeutics to better appreciate coordinated regulation of the hepatocyte phenotype. Copyright 2005 by the Transducer Research Foundation, Inc.

published proceedings

  • Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences

author list (cited authors)

  • King, K. R., Wang, S., Irimia, D., Yarmush, M., Jayaraman, A., & Toner, M.

complete list of authors

  • King, KR||Wang, S||Irimia, D||Yarmush, M||Jayaraman, A||Toner, M

publication date

  • January 2005