Suggested improvements to the standard filter paper assay used to measure cellulase activity. Academic Article

abstract

• Two suggestions can be found in the literature to improve the reproducibility of the Mandels' filter paper assay: add supplemental cellobiase and increase the boiling time for color development. Here we provide data that strongly supports adding supplemental cellobiase. Adding supplemental cellobiase increased assay response by 56%. Cellulases from different sources have different cellobiase activities, which would cause significant variation in the assay response. There is no need for additional boiling time-5 minutes is sufficient. For maximum reproducibility, it is essential that the water bath vigorously boil so that temperature excursions are minimized.

authors

• Holtzapple, Mark

published proceedings

• Biotechnol Bioeng

author list (cited authors)

• Coward-Kelly, G., Aiello-Mazzari, C., Kim, S., Granda, C., & Holtzapple, M.

citation count

• 56

complete list of authors

• Coward-Kelly, Guillermo||Aiello-Mazzari, Cateryna||Kim, Sehoon||Granda, Cesar||Holtzapple, Mark

publication date

• June 2003

publisher

• Wiley  Publisher

published in

• Biotechnology and Bioengineering  Journal

keywords

• Beta-Glucosidase
• Biosensing Techniques
• Cellulase
• Colorimetry
• Enzyme Activation
• Filtration
• Hot Temperature
• Quality Control
• Salicylates
• Sensitivity And Specificity
• Substrate Specificity

PubMed Central ID

• 12673775

Digital Object Identifier (DOI)

• 10.1002/bit.10620

start page

• 745

end page

• 749

volume

• 82

issue

• 6

URL

• http://dx.doi.org/10.1002/bit.10620