Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles. Academic Article uri icon

abstract

  • A method is described for the rapid isolation of microsatellite sequences using a biotin-labeled oligonucleotide attached to streptavidin-coated magnetic particles. The oligonucleotide "hook" in solution hybridizes to complementary single-stranded lengths of genomic DNA onto which have been engineered specific PCR priming sites. The final product is an enriched library of microsatellites of defined sequences. The method is applicable to any genome and in principle is adaptable to the rapid isolation of both repetitive as well as genic sequences. It is illustrated by the isolation of trinucleotide repeat (TAA)n sequences from the citrus genome.

published proceedings

  • Biotechniques

author list (cited authors)

  • Kijas, J. M., Fowler, J. C., Garbett, C. A., & Thomas, M. R.

citation count

  • 253

complete list of authors

  • Kijas, JM||Fowler, JC||Garbett, CA||Thomas, MR

publication date

  • April 1994