Demonstration by 2H ENDOR spectroscopy that myo-inositol binds via an alkoxide bridge to the mixed-valent diiron center of myo-inositol oxygenase. Academic Article uri icon

abstract

  • myo-Inositol oxygenase (MIOX) is a non-heme diiron oxygenase that cleaves cyclohexane-(1,2,3,5/4,6-hexa)-ol (myo-inositol, MI) to d-glucuronate. Here, we use 2H ENDOR spectroscopy to demonstrate that MI binds to the diiron(II/III) cofactor of MIOX via an alkoxide bridge, most likely involving O1. Analysis shows that MI adopts a symmetrical geometry in which the O-C-2H plane of the bridge is approximately orthogonal to the Fe-O-Fe plane.

published proceedings

  • J Am Chem Soc

author list (cited authors)

  • Kim, S. H., Xing, G., Bollinger, J. M., Krebs, C., & Hoffman, B. M

citation count

  • 15

complete list of authors

  • Kim, Sun Hee||Xing, Gang||Bollinger, J Martin||Krebs, Carsten||Hoffman, Brian M

publication date

  • August 2006